Measurement

Part:BBa_K3971020:Design

Designed by: Misaal Bedi   Group: iGEM21_IISER-Pune-India   (2021-10-11)


Promoter characterization cassette for native E.coli csc operon bidirectional promoter.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1478


Design Notes

The blue fluorescent protein and double terminator had to reverse complemented in order to allow transcription in the opposite direction to the yellow fluorescent protein in order to test the activity of the bidirectional promoter in both directions.


Source

The promoter sequence was present in the pCSCX plasmid which was gift from Claudia Vickers (Addgene plasmid # 63918 ; http://n2t.net/addgene:63918 ; RRID:Addgene_63918).

The blue and yellow fluorescent proteins were a part of the iGEM 2021 Distribution Kit along with the double terminator and reversed double terminator.

References